# Endotoxin Quantification Kit for Chromogenic Detection
## Introduction to Chromogenic Endotoxin Quant Kit
The Chromogenic Endotoxin Quant Kit is a powerful tool designed for accurate and sensitive detection of endotoxin levels in various samples. This kit utilizes a chromogenic method that provides reliable results with excellent precision, making it an essential solution for researchers and quality control professionals in pharmaceutical, biomedical, and environmental fields.
## Key Features of the Kit
– High sensitivity with detection limits as low as 0.005 EU/mL
– Wide dynamic range for comprehensive analysis
– Simple and rapid procedure with results in approximately 1 hour
– Excellent correlation with LAL gel-clot method
– Suitable for various sample types including water, pharmaceuticals, and biological fluids
## Principle of Chromogenic Endotoxin Detection
The Chromogenic Endotoxin Quant Kit works based on the enzymatic reaction between endotoxin and Limulus Amebocyte Lysate (LAL). When endotoxin is present, it activates a cascade of enzymes in the LAL reagent, ultimately leading to the cleavage of a synthetic chromogenic substrate. This reaction produces a yellow-colored compound that can be measured spectrophotometrically at 405-410 nm.
## Applications in Various Industries
### Pharmaceutical Industry
The kit is widely used for endotoxin testing in pharmaceutical products, medical devices, and raw materials to ensure compliance with pharmacopeial standards (USP, EP, JP). It helps manufacturers maintain product safety and meet regulatory requirements.
### Biomedical Research
Researchers utilize this kit to measure endotoxin levels in biological samples, cell culture media, and recombinant proteins. Accurate endotoxin quantification is crucial for interpreting experimental results and maintaining cell culture integrity.
### Environmental Monitoring
Environmental scientists employ the Chromogenic Endotoxin Quant Kit to assess endotoxin contamination in water systems, air samples, and industrial environments where bacterial contamination may pose health risks.
## Advantages Over Traditional Methods
Compared to traditional gel-clot methods, the chromogenic assay offers several significant benefits:
Keyword: Chromogenic Endotoxin Quant Kit
– Quantitative results with precise numerical values
– Higher throughput capability
– Reduced subjectivity in interpretation
– Better reproducibility between operators
– Digital record of results for documentation
## Technical Specifications
Parameter | Specification
Detection Range | 0.005-1.0 EU/mL (extendable with dilution)
Incubation Time | 10-16 minutes at 37°C
Sample Volume | 50-100 μL
Readout | Spectrophotometric at 405-410 nm
Kit Components | LAL reagent, chromogenic substrate, control standard endotoxin, buffers
## Conclusion
The Chromogenic Endotoxin Quant Kit represents a significant advancement in endotoxin detection technology, offering researchers and quality control professionals a reliable, sensitive, and efficient method for quantifying endotoxin levels. Its chromogenic detection system provides objective, quantitative results that are essential for maintaining product safety and research integrity across multiple industries.