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# Chromogenic Endotoxin Quantification Kit
## Introduction to Endotoxin Detection
Endotoxins, also known as lipopolysaccharides (LPS), are toxic components found in the outer membrane of Gram-negative bacteria. These molecules can cause severe inflammatory responses when introduced into the bloodstream, making their detection and quantification crucial in pharmaceutical, medical device, and biotechnology industries.
## What is the Chromogenic Endotoxin Quant Kit?
The Chromogenic Endotoxin Quant Kit is a highly sensitive and specific assay designed for the quantitative measurement of endotoxin levels in various samples. This kit utilizes a chromogenic substrate that produces a color change in the presence of endotoxins, allowing for accurate quantification through spectrophotometric analysis.
## Key Features of the Kit
– High sensitivity with detection limits as low as 0.005 EU/mL
– Wide dynamic range for accurate quantification
– Compatibility with various sample types
– Simple and rapid procedure
– Excellent reproducibility
– Compliance with international standards
## Principle of the Assay
The Chromogenic Endotoxin Quant Kit works based on the Limulus Amebocyte Lysate (LAL) reaction. When endotoxin is present in the sample, it activates a cascade of enzymatic reactions in the LAL reagent. The final step of this cascade cleaves a chromogenic substrate, releasing p-nitroaniline (pNA) which produces a yellow color measurable at 405 nm.
## Applications
This versatile kit finds applications in multiple fields:
– Pharmaceutical quality control
– Medical device testing
– Biotechnology product testing
– Water quality monitoring
– Research laboratories
## Advantages Over Traditional Methods
Keyword: Chromogenic Endotoxin Quant Kit
Compared to traditional gel-clot methods, the Chromogenic Endotoxin Quant Kit offers:
– Quantitative rather than qualitative results
– Higher sensitivity
– Better reproducibility
– Faster turnaround time
– Objective measurement through spectrophotometry
## Technical Specifications
Parameter | Specification
Detection Range | 0.005-50 EU/mL
Incubation Time | 10-16 minutes
Sample Volume | 50-100 μL
Wavelength | 405 nm
Storage | 2-8°C
## Conclusion
The Chromogenic Endotoxin Quant Kit provides researchers and quality control professionals with a reliable, sensitive, and efficient method for endotoxin detection. Its chromogenic approach offers significant advantages over traditional methods, making it an essential tool for ensuring product safety in pharmaceutical and medical applications.